Current limitations to high-resolution structure determination by single-particle cryoEM
نویسندگان
چکیده
منابع مشابه
Determination of the ribosome structure to a resolution of 2.5 Å by single-particle cryo-EM.
With the advance of new instruments and algorithms, and the accumulation of experience over decades, single-particle cryo-EM has become a pivotal part of structural biology. Recently, we determined the structure of a eukaryotic ribosome at 2.5 Å for the large subunit. The ribosome was derived from Trypanosoma cruzi, the protozoan pathogen of Chagas disease. The high-resolution density map allow...
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Clathrin triskelions can assemble into lattices of different shapes, sizes and symmetries. For many years, the structures of clathrin lattices have been studied by single particle cryo-electron microscopy, which probed the architecture of the D6 hexagonal barrel clathrin coat at the molecular level. By introducing additional image processing steps we have recently produced a density map for the...
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A single, unique structure of protein determined by X-ray crystal is often used for studies in structure–function relationships. However, the protein is naturally dynamic and fluctuation in solution, the single unique structure is insufficient to illuminate the dynamic character and “personalities” [1,2]. Other than a theoretical calculation approach, such as molecular dynamics simulation is ab...
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In spite of its recent achievements, the technique of single particle electron cryomicroscopy (cryoEM) has not been widely used to study proteins smaller than 100 kDa, although it is a highly desirable application of this technique. One fundamental limitation is that images of small proteins embedded in vitreous ice do not contain adequate features for accurate image alignment. We describe a ge...
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The lethality of anthrax, a zoonotic disease and bioterrorism agent, is due to the anthrax toxin. This tripartitate toxin consists of protective antigen (PA), lethal factor (LF), and edema factor (EF). The 440 kDa heptameric PA prepore binds up to three molecules of LF, a mitogen-activated protein kinase kinase protease, and/or EF, an adenylate cyclase. This entire complex, bound to receptor pr...
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ژورنال
عنوان ژورنال: Quarterly Reviews of Biophysics
سال: 2021
ISSN: 0033-5835,1469-8994
DOI: 10.1017/s0033583521000020